Journal: Frontiers in Molecular Biosciences
Article Title: Multitargeted biological actions of polydatin in preventing pseudogout acute attack
doi: 10.3389/fmolb.2025.1553912
Figure Lengend Snippet: Migration of PBMCs exposed to SFs from patients with CPP induced arthritis in the presence or absence of PD (100–200 µM) or J113866 (10 µM) for 1.30 h. Medium RMPI supplemented with 5% plasma was used as a positive control (C+), while medium RPMI supplemented with 1% FBS was used as a negative control (C-). Left panel : Representative image of migrated cells on the bottom of a filter membrane of a modified 48-well Boyden chamber. Right panel : Effect of 100–200 μM PD on PBMCs migration induced by (A) 5% plasma (n = 3) (B) inflammatory SFs from CIA patients (n = 4). (C) Effect of 10 μM J113863 on PBMCs migration induced by inflammatory SFs from CIA patients (n = 4). Cell migration is shown as optical density values (O.D). Each sample was tested in sextuplicate. Data are expressed as mean of three to four independent experiments ±SD. p calculated according to the One Way Anova, Bonferroni’s multiple comparisons test: *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. CPP, calcium pyrophosphate crystals; PD, polydatin; SF, synovial fluid; PBMCs, peripheral blood mononuclear cells.
Article Snippet: Chemotaxis experiments were performed in a 48-well modified Boyden chamber (Neuro Probe, Gaithersburg, MD) using 5 μm nucleo-pore polyvinyl pyrrolidine-free polycarbonate filters.
Techniques: Migration, Clinical Proteomics, Positive Control, Negative Control, Membrane, Modification